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Alternative analytical approaches include Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS), often coupled with thin layer chromatography (TLC-MALDI) for enhanced separation (Guerrera et al., 2009

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The STEP 4 trial demonstrated that continuous weekly dosing was necessary to maintain weight loss, and discontinuation resulted in weight regain

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