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Immunity 43 , 435449 (2015)

For the quantification of these metabolites in TIF samples, 5 l samples were extracted in 45 l ice-cold extraction solvent (75% acetonitrile, 25% methanol and 0.1% formic acid) containing isotope-labelled internal standards (36 M 13 C 2 , 15 N-GSH, 0.92 M 13 C 4 , 15 N 2 -GSSG and 10 M D 4 -cystine)

Among these, Claudin-5, Occludin, and zonula occludens-1 (ZO-1) are central to maintaining the structural integrity and function of tight junctions (61).Claudin-5, a principal protein in inter-endothelial tight junctions, determines BBB selective permeabilitys (62)

Lotta, L