The NHS and NICE recommend PDE5 inhibitors (sildenafil, tadalafil, vardenafil, avanafil) as first-line treatment, alongside lifestyle modifications and management of underlying conditions
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To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress II One Step Cloning Kit