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Importantly, the ORAC assay uses the area under the curve technique in the presence and absence of the antioxidant

Twin Res

Cells were then incubated with a primary antibody for 12 h at room temperature, followed by the corresponding fluorophore-conjugated secondary antibody (Alexa Fluor) for 45 min and DAPI staining (1 g ml 1 DAPI

For example, the soleus and gastrocnemius muscles of adult males contain 8 and 10 mM carnosine, respectively, or 26.733.3 mmol/kg dry weight of muscle (Derave et al