Commercially available ELISAs were used according to the manufacturers instruction to measure insulin (90080, Crystal Chem), triglycerides (94501, Fujifilm), cholesterol (293-93601, Fujifilm) and free fatty acids (434-91795, 436-91995, 270-77000, Fujifilm)
Drawing on guidance from bodies such as the British Dietetic Association (BDA), the European Society for Clinical Nutrition and Metabolism (ESPEN), and the International Society of Sports Nutrition (ISSN), general ranges are as follows: Sedentary adults: approximately 0.81.0 g of protein per kg of body weight per day Moderately active adults: approximately 1.21.6 g per kg per day Those engaged in regular resistance training: approximately 1.62.0 g per kg per day Older adults (over 65): approximately 1.21.5 g per kg per day, as muscle preservation becomes increasingly important with age (consistent with PROT-AGE and ESPEN consensus) For example, a 90 kg moderately active adult would aim for roughly 108144 g of protein per day
Most of the weight lost with effective incretin therapy is fat, but patients still need a plan to preserve strength, function, and nutritional adequacy
PMID: 25 DengXXieAYeDMaYBaoZXieQet al
This formula provides both the calcium threshold (1,200mg daily recommended) and muscle protection needed during this high-risk period
