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RNA isolation Brain organoids were collected on Day 50 of the protocol

Diaminobenzidine solution (K346811, DAKO, USA) was then added and incubated for 8 min, followed by counterstaining with Harris Hematoxylin, dehydration through graded ethanol to xylene, and coverslipped

CT scan This is a non-invasive X-ray that produces cross-section pictures of the inside of the human body

Effects of high MG levels on the body : Increased amounts of MG lead to problems with mitochondrial function and cause oxidative stress, which disrupts neuron activity

While vitamin B12 can be taken orally, many patients will benefit from injections as they allow the vitamin to be absorbed in a higher proportion than other forms of supplementation