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Connect and filter input water according to manufacturer directions

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PubMed, Ishikawa, Toshihisa, et al

Intended strictly for in vitro research use

In brief, chemical standard libraries of 149 metabolites in seven pooled libraries were prepared and serially diluted in high-performance liquid chromatography (HPLC)-grade water from in a dilution series from 5 mM to 1 M to generate external standard pools, which are used for calibration of isotopically labelled internal standards and to quantify concentrations of metabolites for which internal standards were not available