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Lancet Psychiatry 8 , 969980 (2021)

At this stage of purification GST-X fusion should be clean and depending on the nature of furhter experiments that we want to perform, we can either elute the fusion with glutathione (excess of the glutathione will compete and displace GST-X protein from the beads) or cleave the GST tag and release protein X (see cartoon below)

The decontaminated grains (embryos upwards) were put into Petri dishes (9 cm diameter) on a 0.2% Plant Preservative Mixture (Plant Cell Technologies, Inc., Washington DC, USA)-soaked filter paper and covered

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