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Institutional review board statement All experimental procedures presented in this manuscript were approved and carried out in accordance with the China Veterinary Animal Care Office Animal Experimental Ethics Committee of Guizhou University of Traditional Chinese Medicine (Grant No.20230022)

The PKC pathway becomes activated through hyperglycemia-induced increases in diacylglycerol (DAG), with PKC- and PKC- isoforms particularly implicated in stimulating NOX activity while inhibiting AMPK-mediated antioxidant responses (Paneque et al., 2023)

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This translates to a potential reduction in hyperpigmentation and the appearance of melasma patches