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Fukui M, Song JH, Choi JY, Choi HJ, Zhu BT

2018 Apr;36(2)

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In brief, trypsinized cells were washed three times with PBS and lysed in lysis buffer (5 mM triethanolamine, 150 mM NaCl, 4% SDS, 2 units benzonase, 5 mM PMSF, pH 7.4) with protease inhibitor cocktail followed by adding EDTA to a final concentration of 5 mM