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Patients should return unused vials to the pharmacy or follow local disposal protocols

You lose the synergistic pulse effect that makes the combination valuable

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Transwell assay The chemotactic motility of HUVECs was determined using transwell migration chambers (Corning) with 6.5 mm diameter polycarbonate filters (8 m pore size), as described previously.28 In brief, the bottom chambers were filled with 750 mL of RPMI 1640 medium containing all supplements

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