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doi: 10.1016/j.amjms.2017.12.021 83

The bacteriostatic water should trickle down the inside of the glass wall and pool gradually at the base of the vial, wetting the powder from beneath rather than hitting it from above

In addition to the tissue samples, three QCs were prepared by pooling an equal amount of each experimental sample, and analytical standards of pyruvate, lactate, glucose, fructose-6-phosphate, hydroxybutyrate, leucine, and isoleucine were prepared and analyzed

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