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Post-translational modification analysis For MS analysis of PDHc-E2 or OGDHc-E2 modifications, immunoprecipitation eluates were separated on a pre-cast 4% to 12% NuPAGE gel (Thermo Fisher Scientific, NP0321BOX) and protein bands were stained using SimplyBlue SafeStain solution (Thermo Fisher Scientific, LC6060)

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ONOO- can induce damage to cellular structures, including lipids, proteins, and DNA, leading to oxidative stress and further exacerbating inflammation and tissue injury (149)

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