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Contrary to that, a measurable deficiency rarely occurs under normal physiological conditions and a well-balanced diet, since adipose tissue serves as the main storage and vitamin E can be utilized up to several years [297, 298]
Day 10 cultured SCD erythroblasts were treated with 75 M hemin (#51280, Sigma) (dissolved in 0.25 M NaOH then neutralized with HCL to pH7.4), 50 M dimethyl fumarate (#242926, Sigma) (dissolved in DMSO), 0.5 mM (2S)-Tetrahydro-5-oxo-2-furancarboxylic acid (TFMB-L2HG) (#SML2563, Sigma) (dissolved in DMSO), 0.5 mM S-(5-Adenosyl)-L-methionine iodide (SAM) (#A4377, Sigma) (dissolved in water), and cultured under hypoxia (1% O 2 ) using Heracell 150i incubator equipped with an Oxygen (O 2 ) sensor and Nitrogen (N2) gas supply and analyzed on day 12 of culture
The function of ATPase copper transporter ATP7B in intestine
Funding This work was supported by the Chinese Natural Science Foundation Projects 32072775, 32272915 and 32472949, the National Key Research and Development Programs of China (2023YFD1301003 and 2023YFD1301005), and the Fundamental Research Funds for the Central Universities (2662023DKPY002)