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The most common complaints among these individuals were fatigue, difficulties with concentration (often described as "brain fog"), depression, anxiety, and trouble sleeping

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This alcohol acts as a preservative, which prevents bacteria from growing in the solution

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26,600 g ) for 30 min at 4 C in a JA-25.50 rotor (Beckman Coulter), and cleared lysate was incubated for 45 min with 2.5 mL of Ni-NTA Superflow resin (Qiagen)